Main package methods

Main methods to build signature matrices and deconvolute cell fractions.

deconvolution_methods

List of supported deconvolution methods

build_model()

Building the signature matrix

deconvolute()

Deconvolution

Signature matrix creation methods

Direct access to the individual signature matrix creation methods.

build_model_autogenes()

Calculates the signature model with AutoGeneS

extract_signature_autogenes()

Extraction of the signature matrix from autogenes .pickle files

build_model_bayesprism()

No model is built as BayesPrism performs signature building and deconvolution in one step.

build_model_bisque()

No model is build as Bisque does both steps in one.

build_model_bseqsc()

Calculates the signature model with BSeq-sc

build_model_cibersortx()

Signature matrix creation with CIBERSORTx

build_model_cpm()

No model is build as CPM does not use a signature matrix.

build_model_dwls()

Signature matrix creation with DWLS using genes identified by a differential analysis

build_model_momf()

Calculates the signature model with MOMF

build_model_music()

Signature creation with MuSiC.

build_model_scaden()

Builds Scaden Model from scRNA data

build_model_scdc()

Signature creation with SCDC.

Deconvolution methods

Direct access to the individual deconvolution methods to access special features that are not available through deconvolute.

deconvolute_autogenes()

Deconvolution Analysis using AutoGeneS

deconvolute_bayesprism()

Bayesian deconvolution module using BayesPrism

deconvolute_bisque()

Calculates the decomposition using the bisque algorithm IMPORTANT: No model is needed. Everything is done inside this method.

deconvolute_bseqsc()

Deconvolution Analysis using BSeq-sc

deconvolute_cibersortx()

Deconvolute with CIBERSORTx

deconvolute_cpm()

CPM Deconvolution

deconvolute_dwls()

Calculates the decomposition using the dwls algorithm

deconvolute_momf()

Deconvolution Analysis using MOMF (via Nonnegative Factorization)

deconvolute_music()

MuSiC Deconvolution

deconvolute_scaden()

Performs deconvolution with Scaden

deconvolute_scdc()

SCDC Deconvolution

Environment

Methods needed to set credentials or change variables in the environment.

set_cibersortx_credentials()

Setting the CIBERSORTx Credentials

bseqsc_config()

Setup BSeq-SC External Dependencies

Datasets

List of datasets provided by the package.

bulk

Example RNA-seq dataset from Hoek et al.

RefData

Gold standard measurements with FACS from Hoek et al.

cell_type_annotations_1

Cell Type Annotations Number 1 from Hao et al.

cell_type_annotations_2

Cell Type Annotations Number 2 from Hao et al.

cell_type_annotations_3

Cell Type Annotations Number 3 from Hao et al.

single_cell_data_1

Example single-cell RNA-seq dataset Number 1 from Hao et al.

single_cell_data_2

Example single-cell RNA-seq dataset Number 2 from Hao et al.

single_cell_data_3

Example single-cell RNA-seq dataset Number 3 from Hao et al.

batch_ids_1

Patient ids Number 1 from Hao et al.

batch_ids_2

Patient ids Number 2 from Hao et al.

batch_ids_3

Patient ids Number 3 from Hao et al.

Visualisation

Methods which produce plots with different methods.

make_benchmarking_scatterplot()

Make a Scatterplot for Benchmarking

make_barplot()

Plot deconvolution results as a barplot

plot_deconvolution()

Plot Deconvolution results